Effect of Combination of Tween-80 with Amphiphilic Peptides on Physical Stability of Nanoemulsion Using Low-Energy Emulsification Technique

Abstract

The current study was investigated to replace Tween-80 with peptides in order to produce physical and microstructure stability of nanoemulsion. Oil phase (2%), Tween-80 (4%) and remaining aqueous phase were taken to form nanoemulsions. In combinations of Tween-80 and peptides three ratios (1:2, 2:1 and 1:1) were taken keeping the overall concentration 4% fixed. The particle size of nanoemulsion stabilized with Tween-80, peptides and combination of Tween-80-peptides (1:2, 1:1, 2:1) were 148.71 ± 1.48, 283.66 ± 10.17, 254.50 ± 14.77, 161.90 ± 3.67, 182.80 ± 5.37 nm, showing smallest particle size with 50% replacement of Tween-80 with peptides. The zeta potential data showed competitive adsorption between Tween-80 and peptides. The polydispersity index of Tween-80:peptides (1:1) had found to be 0.33 ± 0.01 where as Tween-80 and peptides stabilized nanoemulsion had 0.20 ± 0.01 and 0.30 ± 0.05. The changes in electrical conductivity occurred maximum in case of peptides and electrical conductivity decreased with increase in concentration of Tween-80 due to preventing mobility of ions. The storage stability of nanoemulsions were found better at 4 ºC, however Tween-80:peptides (1:1) and peptides stabilized nanoemulsion had particle size growth onwards 14 days at 37 ºC. The transmission electron microscopy (TEM) image of nanoemulsion Tween-80:peptides (1:1) showed uniform distribution of peptides in combination compared with individual peptides stabilized nanoemulsion.