Effect of chloretracycline and albendazole on alkaline phosphatase activity of carp gill (Cyprinus carpio L.)

I Kurbatova,O Yaremchuk,M Zakharenko

doi:10.15421/2020_196

Abstract

The effect of the antibiotic chlortetracycline and the anthelmintic albendazole on morphological features, internal organs, alkaline phosphatase activity and protein content in the gills of two-year-old carp was studied. It was found that chlortetracycline and albendazole at the studied concentrations in water and short-term action do not affect the morphological features, behavior and internal organs, but change the alkaline phosphatase activity of the gills of two-year-old carp. The antibiotic chlortetracycline at concentrations of 1.10, 3.15, and 6.30 mg/dm3 of water and fish exposure for 72 h reduced the alkaline phosphatase activity of gills in carp of the first experimental group by 2.5 times, in the second – by 2.8 times and in the third – by 2.7 times regard the control; it did not affect the protein content in the gills, morphological features and internal organs of fish. We registered an increase in alkaline phosphatase activity of gills in two-year-old carp under effect of anthelmintic albendazole at at the concentration of 1.0 mg/dm3 by 28.2%, while albendazole concentration of 0.2 and 0.5 mg/dm3 did not affect the alkaline phosphatase activity as well as the level of protein in fish body. Our results indicate an important role of gill alkaline phosphatase in assessing the negative effects of antibiotics and water anthelmintics in freshwater fish.

Highlights

Livestock enterprises and veterinary facilities have a significant impact on the functioning of aquatic ecosystems (Bártíková et al, 2015; Tertychna et al, 2017; Prichepa et al, 2019)
The aim of the study was to investigate the effect of the antibiotic chlortetracycline and the anthelmintic albendazole on alkaline phosphatase activity and protein content in the gills, morphological features and internal organs of carp (Cyprinus carpio L.) biennials
It was shown that the alkaline phosphatase activity of carp gills under the action of chlortetracycline was reduced in fish of the first experimental group by 2,5 times compared with the control

Summary

Introduction

Livestock enterprises and veterinary facilities have a significant impact on the functioning of aquatic ecosystems (Bártíková et al, 2015; Tertychna et al, 2017; Prichepa et al, 2019). Excreted from the body of animals almost unchanged, antibiotics and anthelmintics accumulate in wastewater, are not removed during treatment at treatment plants and fall into natural bodies of water (Carvalho, 2016; Mehed et al, 2019; Kovalakova et al, 2020) This creates risks of antibiotics entering the food chains of water bodies and drinking water, which provides for constant monitoring of their presence and content in the wastewater of livestock enterprises (Bengtsson-Palme et al, 2018; Rodriguez-Mozaz et al, 2020). The action of these xenobiotics of water on fish is associated with the impact on the gills, morphological features, internal organs, immune system, hematopoiesis and metabolism in organs and tissues (Kurbatova et al, 2018; Li, 2019; Grenni et al, 2018). The aim of the study was to investigate the effect of the antibiotic chlortetracycline and the anthelmintic albendazole on alkaline phosphatase activity and protein content in the gills, morphological features and internal organs of carp (Cyprinus carpio L.) biennials

Objectives

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Results

Conclusion