Effect of Bone Morphogenetic Protein-4 on the Expression of BMP-7In Bone Repair

Abstract

Background: BMPs play important roles in normal bone development and regulation of bone repair by their ability to stimulate osteogenesis,cell differentiation and apposition of bone matrix. Aim of study: to find role for the application of BMP4 in bone defect with expression of BMP7 by newly formed bone. Materials and Method: Twenty male Wistar rats were used to create a drill-hole injury (3mm) bone defects in femur ,one in the left side represented the control ,while the right side represented the experimental. The study groups include: z Control group,the bone defect was left without treatment . z Experimental group ,the bone defect treated with 0.5 ?l of BMP4. Bone repair was examined histologyically and immunohistochemically for the expression of BMP7 at the postoperative periods 14 and 28 day . Result: Show increased bone formation with proceeding time postoperatively for both study groups,although experimental group illustrates more bone apposition that filled the defect at 28 day.Moreover intense positive expression of BMP7 by stromal and bone cells were illustrated in experimental group with high significant differences in comparisum to control. Conclusions: BMP4 seems to play an important role in bone repair; induced expression of BMP7 correlates with the bone repair outcome.