Effect of Argon Plasma on Root Dentin after Use of 6% NaOCl.

Maíra Do Prado,Laura Villela Pacheco,Renata Antoun Simão,Carlos Augusto De Melo Barbosa,Rafael Nigri Roizenblit,Carolina Oliveira De Lima

doi:10.1590/0103-6440201600486

Maíra Do Prado, Laura Villela Pacheco + Show 4 more

Open Access

https://doi.org/10.1590/0103-6440201600486

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Abstract

The aim of this study was to evaluate the effect of argon plasma on dentin surface after use of 6% NaOCl. Sixty bovine incisors had their crowns removed, the roots split, and the segments planed. One hundred twenty segments of the cervical third were used. The samples were divided in two groups (n=60): immersed in 6% NaOCl, washed, dried and then immersed in 17% EDTA, washed and dried and Argon group: after treatment described for the CONTROL GROUP, non-thermal argon plasma was applied for 30 s. Ten samples were evaluated by scanning electron microscopy in each group. Other ten samples were analyzed by Fourier transform infrared spectroscopy (FTIR). Thirty samples were analyzed with a goniometer to measure the contact angle between the dentin surfaces and solutions, to determine the surface free energy. The last ten samples were used to evaluate the wettability of AH Plus sealer. Data were statistically analyzed using Kruskal Wallis and Mann-Whitney tests (p<0.05). The results of this study showed that argon plasma did not modify the surface topography. FTIR analysis showed chemical modifications after plasma treatment. Argon plasma increased the surface free energy of dentin and AH Plus wettability. In conclusion, argon plasma treatment modified chemically the dentin surface. This treatment increased the surface free energy and wettability of an epoxy resin root canal sealer, favoring its bonding to dentin surfaces.

Highlights

Effective cleaning and shaping of the root canal, and creating a coronal and apical seal are essential goals for successful endodontic treatment and prognosis [1,2]
Among the auxiliary chemical substances used in endodontics during chemical-mechanical preparation, sodium hypochlorite, in different concentrations, has been most commonly used
This endodontic irrigant is able to destroy a broad spectrum of microbes and to dissolve organic tissue; it is known that sodium hypochlorite solutions have cytotoxic effects and modify the organic components of dentin, especially collagen

Summary

Introduction

Effective cleaning and shaping of the root canal, and creating a coronal and apical seal are essential goals for successful endodontic treatment and prognosis [1,2]. Among the auxiliary chemical substances used in endodontics during chemical-mechanical preparation, sodium hypochlorite, in different concentrations, has been most commonly used. This endodontic irrigant is able to destroy a broad spectrum of microbes and to dissolve organic tissue; it is known that sodium hypochlorite solutions have cytotoxic effects and modify the organic components of dentin, especially collagen. Depending on the plasma chemistry or gas composition, these highly reactive plasma species can react with, clean and etch surface materials, bond to various substrates, or combine to form a thin layer of plasma coating, and modify the surface characteristics [7]. Argon plasma was indicated mainly to improve the adhesion process of resinbased materials to dentin [13]

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