Abstract
Objective To investigate the effect of arginine-modified chitosan gene nanoparticles(ACGN)on the change of platelet GMP-140. Methods Arginine-modified chitosan(ACS) was synthesized and characterized by FTIR. ACGN were prepared by coacervation process. Interaction between ACS and DNA was investigated by agarose gel electrophoresis retardation assay. GMP-140 ELISA kits were used to investigate the effect of arginine-modified chitosan gene nanoparticles on the change of platelet GMP-140 in vivo and in vivo. Results FTIRresult demonstrated that arginine was successfully incorporated into the backbone of chitosan. Plasmid DNA was entirely entrapped by ACS at the charge ratio of 2:1 or above. Platelet activation wasn't caused by CGN or ACGN in vitro, but slight platelet activations were caused by both CGN and ACGN in vivo, as compared with the control group. Conclusion ACS didn't cause obvious change in platelet GMP-140, which suggested that ACS would be a novel and safe non-viral vector. Key words: Chitosan; Gene nanoparticles; GMP-140
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