Abstract
Objective To investigate the effect of arginine-modified chitosan gene nanoparticles (ACGN) on complement activation and hemolyzation. Methods Arginine-modified chitosan (ACS) was synthesized by incorporating arginine onto chitosan backbone. ACGN were prepared by coacervation process. Interaction between ACS and DNA was investigated by agarose gel electrophoresis retardation assay and photon correlation spectroscopy(PCS). ELISA kits were used to investigate the effect of ACGN on complement activation and hemolyzation in vivo and in vivo. Results ACS can entrap whole plasmid DNA at charge ratio of 2:1 and formed nanoparticles with mean diameters about 120-180 nm. ACGN didn't play an obvious detrimental effect on red blood cells in vivo and intro. ACGN had no obvious influence on complement activation in vitro, but caused a slight increase in vivo. Conclusion ACGN didn't cause obvious change in complement activation and hemolyzation, which suggested that ACS would be a novel and safe non-viral vector. Key words: Chitosan; Gene nanoparticles; Complement activation; Hemolyzation
Published Version
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