Abstract
Glucans are a type of polysaccharide found in the cell walls of plants, bacteria and yeasts. β-glucans have various biological activities as antitumour, antioxidant, anticholesterol, antiageing and immune system booster known as an immunomodulator. One of the yeasts that can produce β-glucans is Saccharomyces cerevisiae because most of the cell wall structures contain mannoproteins, β-1,3-glucan, and β-1,6-glucan, which function to strengthen cell structure and as a portion reserve food. This study aims to see the effect of adding ammonium chloride and disodium chloride with molasses as a natural substrate for the growth of Saccharomyces cerevisiae in the production of β-glucose. The research stages began with making a growth curve, fermentation process, separation of β-glucan, and qualitative and quantitative analysis. Qualitative analysis showed positive results for β-glucan. In quantitative analysis, the highest amount of cell biomass obtained in Formula 4 = (04395 ± 0,1318) mg containing 4% molasses, 2% peptone, 0,2% Ammonium chloride and 0.02% Na2HPO4 and distilled water up to 150 mL with a fermentation time of 72 hours. The addition of ammonium chloride and disodium hydrogen phosphate affected beta-glucan levels after 72 hours of fermentation in Formula 4 = (0,0840 ± 0,0467) %.
Published Version
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