Effect of agar concentration in culture medium on in vitro development of potato plants

Cinthia Kutz De Matos,Camila Eduarda Loli Pereira,Jackson Kawakami,Leonardo Balena

doi:10.33448/rsd-v9i7.4571

Cinthia Kutz De Matos, Camila Eduarda Loli Pereira + Show 2 more

Open Access

https://doi.org/10.33448/rsd-v9i7.4571

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Abstract

In vitro propagation and tissue culture technique is an alternative for obtaining healthy potato seeds. However, data found in the literature differ on the optimal consistency of the culture medium to be used in vitro. The aim in this research was to look at the adaptation of potato cultivars to medium with different agar concentrations. The experimental design was entirely randomized in a factorial scheme. The experiment tested the effect of 3 agar medium concentrations (solid, semisolid and liquid) on 6 cultivars (Agata, Bel, Camila, Catucha, Clara and Eliza), with 5 repetitions. We quantified from the plantlets, fresh and dry mass of roots and shoot, the number of nodes, the multiplication capacity and vigor. The study showed that potato cultivars respond significantly to different concentrations of agar and their best response was in the semisolid medium.

Highlights

Potatoes (Solanum tuberosum L.) have high social and economic importance, corresponding to the fourth most produced food in the world, surpassed only by wheat, corn, and rice (FAOSTAT, 2020)
In vitro propagation and tissue culture technique is an alternative for obtaining healthy potato seeds
There was a production of about 368 million tons of potato on 17.5 million hectares in the world in 2018 (FAOSTAT, 2020), of which around 3.7 million tons were produced in Brazil on 118 thousand hectares (IBGE, 2020)

Summary

Introduction

Potatoes (Solanum tuberosum L.) have high social and economic importance, corresponding to the fourth most produced food in the world, surpassed only by wheat, corn, and rice (FAOSTAT, 2020). Tissue culture is an alternative to obtaining high health seeds, the technique consists of aseptically cultivating cells, tissues or organ fragments of a given plant in artificial culture medium under a controlled environment, aiming at the development of new plants (Morais et al, 2018). This cultivation method is characterized by speed and a high rate of multiplication in a short time (Mohapatra & Batra, 2017)

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