Abstract
Amino acid incorporation into protein by liver ribonucleoprotein particles and kidney microsomes, obtained from normal and adrenalectomized rats, was investigated. The liver and kidney particles were prepared at various times, ranging from 3 h to 31 days after adrenalectomy. The 14C-incorporating activity of liver particles from adrenalectomized rats was greater than that observed with particles from liver of normal animals up to 15 days after adrenalectomy; at 21 and 31 days after the operation the liver particles showed an activity 60 and 80 %, respectively, of that of the control group. Microsomes from kidney of adrenalectomized rats demonstrated an incorporating activity higher than that of the microsomes from kidney of normal animals at all the times investigated; a sharp increase was observed at 21 and 31 days after adrenalectomy, when the activity of the liver particles was low. A correlation was established between incorporating activity of liver and kidney particles; to an increase or decrease in the activity of the particles from the liver corresponded a decrease or an increase, respectively, in the activity of the kidney microsomes. The 14C-incorporating activity of both liver and kidney particles was directly proportional to the ratio of μg RNA to mg protein in these particles up to 15 days after adrenalectomy, deviations occurring at more prolonged time after the operation.
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