Effect of Abnormal Savda Munziq, a Traditional Uighur Herbal Medicine, on Pulmonary Function and Aquaporins of COPD Rat Model with Abnormal Savda Syndrome.

Wang Jing,Jing Jing,Gao Zhen,Xu Dan,Li Zheng,Halmurat Upur,Li Fengsen

doi:10.1155/2017/7176263

Abstract

Objective To investigate the effect of abnormal savda munziq (ASM) on the pulmonary function and expression of lung-specific aquaporins in the rat model of chronic obstructive pulmonary disease with abnormal savda syndrome (ASSCOPD). Methods Eighty male rats were randomized into ASSCOPD, COPD, and control groups. ASSCOPD was further categorized into ASM and non-ASM groups. COPD model was established by combining fumigation with airway instillation of elastase; ASSCOPD model was developed based on COPD by induction with dry cold diet, cold dry environment, and plantar electric stimulation. ASM was administered twice daily. The pulmonary function was evaluated based on respiration. The mRNA and protein levels of AQPs were estimated by real-time PCR and Western blot, respectively. Results MV, TV, the mRNA level of AQP5, and the protein expression of AQP1, AQP4, and AQP5 were increased in ASMCOPD compared to ASSCOPD. Conclusion The pulmonary function was impaired in ASSCOPD group; the expression of AQP1, AQP4, and AQP5 was decreased at protein and mRNA levels in ASSCOPD group. ASM can improve the pulmonary function in ASSCOPD for MV and TV. ASM could elevate the protein expression of AQP1, AQP4, and AQP5 and the mRNA level of AQP5 in lung tissue.

Highlights

Chronic obstructive pulmonary disease (COPD) is a preventable and treatable respiratory disease characterized by airflow limitation; the restricted airflow cannot be completely reversed, and it continuously progresses if not effectively controlled
A 5 mL abnormal savda munziq (ASM) solution was administrated through gavage twice a day, each morning and evening, and the rats from the ASSCOPD, COPD, and control groups were treated with an equivalent volume of normal saline for 7 days
Airway mucus hypersecretion led to the increased airway mucus viscosity and increased airway resistance, which adversely affected the clearance of mucus resulting in the residence and proliferation of pathogenic bacteria in the respiratory airway, aggravating the infection

Summary

Introduction

Chronic obstructive pulmonary disease (COPD) is a preventable and treatable respiratory disease characterized by airflow limitation; the restricted airflow cannot be completely reversed, and it continuously progresses if not effectively controlled. The incidence of COPD was reported as 8.2% in the cohort of 40year-old or more senior populations in China. The data were collected from seven cities and provinces [1]; the incidence of COPD in South Korea was 13.4% from a similar cohort [2]. COPD had been considered to be one of the leading causes of disability and deaths globally, bringing enormous economic and social burden [3]. The effective prevention and treatment of COPD continue to remain a vital public health concern to be resolved urgently

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Conclusion