Correlative analysis of neoplasm patients with phlegm-stasis () or abnormal savda () syndrome, based on metabonomics

Abstract

ObjectiveTo investigate plasma samples from neoplasm patients with phlegm-stasis or abnormal Savda syndrome, with NMR spectroscopy, and to analyze their metabolic varieties, characteristics and reciprocity. Methods1H-NMR spectra were analyzed using the orthogonal projection to latent structure with discriminative analysis (OPLS-DA) method with unit variance scaling. The discriminative significance of metabolites was determined by the Pearson's product–moment correlation coefficient. ResultsCompared to the control group, neoplasm patients with phlegm – stasis or abnormal Savda syndrome had low concentrations of leucine, isoleucine, valine, alanine, tyrosine, histidine, citrulline, glycoprotein, glutamine, myo-inositol, scyllo-inositol, creatine, α-glucose, β-glucose and lactate (P<0.05), and high concentrations of very low density lipoprotein, low density lipoprotein, unsaturated lipid, formate, acetone, acetate, acetoacetate, pyruvate, β-hydroxybutyrate, carnitine and malonic acid (P<0.05), with no significant differences between the phlegm – stasis and abnormal Savda syndrome patients. ConclusionsNeoplasm patients with different syndromes have very similar metabolic changes. A series of abnormalities such as immune dysfunction and oxidative – antioxidative imbalance, occur in neoplasm patients with abnormal Savda or phlegm – stasis syndrome.