Abstract
Objective To investigate the changes of pulmonary surfactant (PS) in acute lung injury (ALI) induced by oleic acid and the intervention effect of β-adrenergic receptor agonists, and to provide a theoretical basis and practical direction for new therapeutic measures in the treatment of ALI. Methods Thirty Sprague-Dawley rats were randomly divided into ALI group induced by oleic acid (OA group, n = 10) , isoprenaline treatment group (ISO group, n =10) , and normal saline control group (NS group, n = 10). After four hours, PaO2 , PaCO2, pH, and the serum surfactant protein A (SP-A) levels were assayed. After right lung was lavaged, total proteins ( TP ), total phospholipids ( TPL), phosphatidylcholine (PC) and SP-A in the bronchoalveolar lavage fluid (BALF) were determined. The left lung was used for measurement of wet/dry weight ratio (W/D), beta-adrenergic receptor (β-AR) ,and histopathology. Results Compared with NS group, TPL, PC/TPL and SP-A in the BALF significantly decreased (all P ＜0. 01), W/D, TP and serum SP-A were increased ( P ＜0. 05 or P ＜0.01) in OA group. The values of SP-A in the BALF were negatively correlated to those in the serum in OA group ( r = - 0. 910, P ＜0. 05). Compared with OA group, TPL, PC/TPL and SP-A in the BALF increased, W/D, TP and serum SPA decreased in ISO group ( P ＜0. 05 or P ＜0. 01). Conclusions The PS inactivation may be an important internal cause during the course of ALI induced by oleic acid. The serum SP-A concentration is significantly elevated in ALI rats, so serum SP-A may be a marker for the early diagnosis of ALI, and β-adrenergic receptor agonists may play some protective role in the early stage of ALI. Key words: Acute lung injury; Oleic acid; Pulmonary surfactant; β-adrenergic receptor agonists
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call