Działanie olejku ylangowego (Cananga oil) wobec grzybów drożdżopodobnych

Abstract

Introduction. Several herbs produce substances with medicinal properties. They show antioxidant, antiinflammatory and antimicrobial activity. Cananga odorata Hook (Annonaceae family) is found in the Philippines, Madagascar island, Sumatra, in Jemen and Australia. It is fast growing, evergreen tree, that reaches up to 20 m. Essential oil produced of flowers is used in medicine. The chemical composition of the Cananga oil is as follows: geraniol, linalool, methyl salicylate, α-terpineol, eugenol, α- and β-caryophyllene, farnesen, δ-cadinene, γ-kadinene, geranyl acetate, methyl p-cresyl ether, p-cresol, neridol, α-pinene, carbohydrates, saponins, tanins, flavonoids, amino acids and coumarins. It possess different therapeutic properties. Aim. The aim of the study was evaluation of antifungal activity of Cananga oil. Material and methods. The strains of fungi were isolated from oral cavity. They were from the following genera: Candida albicans (10 strains), C. glabrata (4), C. guilliermondii (2), C. kefyr (2), C. krusei (4), C. lusitaniae (2), C. parapsilosis (3), C. tropicalis (5) and C. utilis (2). Furthermore 9 reference strains C. albicans ATCC 10231, C. glabrata ATCC 66032, C. guilliermondii ATCC 6260, C. kefyr ATCC 4130, C. krusei ATCC 14249, C. lusitaniae ATCC 34499, C. parapsilosis ATCC 22019, C. tropicalis ATCC 750 and C. utilis ATCC 9958 were tested. The sensitivity (MIC) of the yeast to Cananga oil (Semifarm) was determined by method of plate dilution technique in Sabouraud’s agar. First the essential oil was dissolved in dimethyl sulfoxide (DMSO, Serva) and then in sterile distilled water. The oil concentrations were: 15.0, 10.0, 7.5, 5.0, 2.5 and 1.2 mg/ml. The inoculum containing 105 microorganisms per drop (CFU) was applied with Steers replicator on agar with or without oil (strains growth control). Incubation of the agar plates was carried out under aerobic conditions at 37°C for 12-48 hrs. The MIC was defined as the lowest concentration of the Cananga oil inhibited the growth of the tested strains of yeast-like fungi. Results. The results show, that the growth of 97% of the tested strains of yeast-like fungi was inhibited in the concentration range 1.0-10.0 mg/ml. The strains of C. albicans and C. glabrata were susceptible in range 2.5-5.0 mg/ml. The oil shoved activity against C. guilliermondii and C. utilis strains at a concentration of 5.0 mg/ml. Only 1 strain of C. parapsilosis and 1 strain of C. tropicalis were susceptible at 1.2 mg/ml. However, the oil shoved the lowest activity against C. krusei strains. The MIC values of Cananga oil ranged from 10.0 to 15.0 mg/ml. Conclusions. Most of the estimated strains from Candida genus were susceptible to low concentration of Cananga oil. The oil in low concentrations inhibited the grows of C. albicans strains. The C. krusei strains were the least sensitive to Cananga oil.