Dynamics of indicators of cellular immunity in conditions of acute generalized peritonitis in rats

Abstract

The course of peritonitis depends on the state of the immune system and the adequacy of the immune response. The aim of this investigation was to explore the features of the cellular immune response cell in rats with simulated acute generalized peritonitis (AGP). The study was conducted on 32 rats, divided into two groups: the main group – 24 animals with simulated peritonitis; control– 8 intact animals. In the animals of the main group AGP were modeleted by injecting of 10 % filtered stool suspension into the abdominal cavity of the study rats at a dose of 0.5 ml per 100 g of body weight. Removal of material for histological examination was carried out on days 1, 3 and 7. Indicators of cellular immunity were determined by a method that was based on the interaction of fluorescently labeled monoclonal antibodies with lymphocyte surface antigens. In the main group of animals, all indicators of the cellular immunity gradually decreased from 1 to 7 days of the experiment. The concentration of CD3+ - cells decreased by 1.90 times per day, by 1.97 times by 3 days, and by 2.10 times by 7 days, compared with intact animals. Suppression of the cellular immunity was observed after modeling of AGP, which is combined with a decrease in the number of both CD3+ lymphocytes and the main subpopulations of CD4+, CD8+ and CD16+ cells. The greatest decrease in the cellular level of immunity was observed on day 7 of the experiment.