Morphological Characteristics of Lungs with Experimental Peritonitis on the Background of Diabetes Mellitus

Abstract

Acute generalized peritonitis (AGP) and diabetes mellitus (DB) remains an urgent problem in modern medicine, as rapid disability and high mortality have been leading to cardiovascular disease and oncology pathology in recent years. The purpose is to study and evaluate the morphological changes in the lungs in animals with experimental AGP against the background of DM. Іn the experiment, 32 white rats were used. The main group consisted of 24 animals with simulated AGP against the background of CD; control group – 8 intact animals. The experimental CD was reproduced by intraperitoneal administration of streptozotocin at a dose of 60 mg/kg. On the 14th day after administration of streptozotocin, animals of the main group were injected with 10 % filtered stool suspension into the abdominal cavity of the study rats at a dose of 0.5 ml per 100 g of body weight. Removal of material for histological examination was carried out on days 1, 3, and 7. For the morphological study of the lungs of rats under experimental peritonitis, the method of light microscopy was used. Micropreparations were stained with hematoxylin and eosin, according to Hart, by the Van Gizon method. Histological examination of the lung tissue of animals with simulated AGP against the background of CD compared with intact rats on day 1 of the experiment showed a tendency for vasodilation. For 3 days, the lungs of the organs were observed in the lungs of animals; the wall was thickened in the vessels of large and medium caliber, the phenomena of mucoid edema and perivascular edema with small cell infiltration were noted. At day 7, significant changes in blood vessels and bronchi in all components were detected in the lungs of animals; Perivascular edema and inflammatory infiltration along the interval septa were observed in animals with simulated AGP against the background of CD in comparison with intact rats for 1 day of the experiment in lung tissue. The wall of small bronchi was thickened by leukocyte infiltration. For 3 days, the lungs of the animals were observed full blood vessels of the pulmonary components, the phenomena of mucoid edema were noted. For 7 days in the lungs of animals found the expansion of the lumen of the arteries and veins from their full blood, thickening of the outer shell of the vessel wall.