Abstract
To meet the present and forecasted market demand, bacterial alkaline phosphatase (ALP) production must be increased through innovative and efficient production strategies. Using sugarcane molasses and biogenic apatite as low-cost and easily available raw materials, this work demonstrates the scalability of ALP production from a newfound Bacillus paralicheniformis strain APSO isolated from a black liquor sample. Mathematical experimental designs including sequential Plackett–Burman followed by rotatable central composite designs were employed to select and optimize the concentrations of the statistically significant media components, which were determined to be molasses, (NH4)2NO3, and KCl. Batch cultivation in a 7-L stirred-tank bioreactor under uncontrolled pH conditions using the optimized medium resulted in a significant increase in both the volumetric and specific productivities of ALP; the alkaline phosphatase throughput 6650.9 U L−1, and µ = 0.0943 h−1; respectively, were obtained after 8 h that, ameliorated more than 20.96, 70.12 and 94 folds compared to basal media, PBD, and RCCD; respectively. However, neither the increased cell growth nor enhanced productivity of ALP was present under the pH-controlled batch cultivation. Overall, this work presents novel strategies for the statistical optimization and scaling up of bacterial ALP production using biogenic apatite.
Highlights
The bioeconomy represents the value chain of sustainable manufacturing using renewable, low-cost biological resources to sustainably produce food, energy, and industrial products[1]
To screen alkaline phosphatase (ALP)-producing isolates from the obtained isolates, the plate agar media assay used an artificial substrate that yielded a colored product like the yellow color of the para-nitrophenol end product as a result of para-nitrophenyl phosphate cleavage and a deep-green color as a result of cleavage of phenolphthalein diphosphate tetrasodium salt (PDP) in presences of methyl green (MG) as an indicator dye, as detailed by Patel Falguni and o thers[14] for selecting phosphatase producers
H2O, 0.0005) allowed for a 21-fold increase in productivity. These results demonstrate the first such optimization of ALP production employing animal bone powder as a biogenic apatite source
Summary
The bioeconomy represents the value chain of sustainable manufacturing using renewable, low-cost biological resources to sustainably produce food, energy, and industrial products[1]. Designing effective and cost-competitive green technologies capable of sustainably producing bioproducts from biomass is a key challenge facing a rising bioeconomy. Green chemistry is an eco-friendly approach that maintains sustainability by efficiently using raw materials, eliminating waste, and avoiding the use and generation of substances toxic or hazardous to human health and the environment in the manufacturing and application of biobased p roducts[4]. Industrial sugar production from beets and sugarcane generates molasses, an opaque, nutrient-rich liquid, as a byproduct, providing a renewable, reliable, readily available, and low-cost raw material that can be used to feed most microorganisms, as it contains carbon, Scientific Reports | (2021) 11:6071. Enzymatic bioremediation is a vital branch of green chemistry used to clean contaminated sites in which microorganisms enzymatically attack recalcitrant environmental pollutants and break them down or convert them to innocuous p roducts[7]
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