Drug dissolution studies in milk using the automated flow injection serial dynamic dialysis technique

Abstract

The application of flow injection serial dynamic dialysis (FISDD) technique to monitor dissolution studies in complex media is described. The method is based on the study of the kinetics of the simultaneous dissolution and dialysis processes. Commercial formulations of salicylamide, propantheline bromide, nitrofurantoin and acetaminophen were used to investigate the utility of the FISDD technique for studying the dissolution of drugs in low fat milk. The latter was utilized as a food simulating medium. Dissolution studies were also conducted in phosphate buffer of pH 6.5. In each case the FISDD system was coupled with the rotating basket apparatus. The determination of the dialyzable drugs was performed automatically by the FIA analyzer. A fully automated monitoring of dissolution of drugs in milk and buffer was achieved. In all cases the dissolution rate of drugs in milk was lower than the corresponding rate in the aqueous buffer. The potential significance of this system with respect to the in vitro study of dissolution of drugs in food simulating media is discussed. This system could be used either to reveal or explore food-drug and/or food-formulation interactions anticipated or observed in vivo.