Abstract
Here, we report the annotated draft genome sequences of three Pseudomonas spp. and two Bacillus spp. that, as consortia, degrade polyethylene terephthalate plastic. Improved microbial degradation of plastic waste could help reduce the billions of metric tons of these materials that currently exist in our environment.
Highlights
We report the annotated draft genome sequences of three Pseudomonas spp. and two Bacillus spp. that, as consortia, degrade polyethylene terephthalate plastic
Cultures of the five individual strains were grown overnight in LB broth and diluted to an optical density at 600 nm (OD600) of 1 to ensure that equal amounts of bacteria were added to each sample
Aliquots of each overnight culture were washed with phosphate-buffered saline twice, and equal volumes of bacteria were added to the appropriate 10-ml culture of liquid carbon-free basal medium (LCFBM) with 0.1 g total Polyethylene terephthalate (PET)
Summary
We report the annotated draft genome sequences of three Pseudomonas spp. and two Bacillus spp. that, as consortia, degrade polyethylene terephthalate plastic. León-Zayas et al FIG 1 The full consortium, including all five strains, degrades PET to a greater extent than does any individual isolate or individual consortium after a 6-week incubation. Cultures of the five individual strains were grown overnight in LB broth and diluted to an optical density at 600 nm (OD600) of 1 to ensure that equal amounts of bacteria were added to each sample. Aliquots of each overnight culture were washed with phosphate-buffered saline (pH 7.4) twice, and equal volumes of bacteria were added to the appropriate 10-ml culture of LCFBM with 0.1 g total PET (starting OD600, 0.02).
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