Studying the inhibitory effect of local honey, black seed oil against some bacterial isolates with a comparisons

Abstract

The current research aimed to investigate the antibacterial activity of two types of honey alone and in combination with black seed oil and compare their effects. The first type of honey, the mountain type, was obtained from some beehive of Sulaymaniyah city, while the second type (Lowlander) was obtained from beehive in Balad city of Salah Al-Deen. The bacterial species used were Staphylococcus aureus, Escherichia coli, Salmonella typhi and Proteus mirabilis categorized into clinical and environmental groups. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Different concentrations were conducted ranged from 20-100%. Results had revealed that the environmental bacterial isolates were affected mostly toward both honey types. However, the mountainous honey showed inhibitory effect against the Gram negative isolates. Mixture of honey in general with black seed oil had significantly differed and at some points decreased the effect while the black seed alone showed effect toward the environmental isolates at the absolute concentration.