Inhibitory effect of Titanium dioxide (Tio2) nanoparticles and their synergistic activity with antibiotics in some types of bacteria

Abstract

Titanium dioxide nanoparticles (TiO2 NPs) were studied as antibacterial agents at different concentrations against clinical and environmental bacterial isolates without UV or photocatalytic activation. Five TiO2 NPs concentrations (20µg/ml,50µg/ml, 100µg/ml,500µg/ml and 1000µg/ml) were studied against 15 bacterial species:10 clinical isolates and 5 environmental isolates) compared with antibiotics Amikacin(AK)and Levloxacin(LEV).Only500µg/ml concentration of TiO2 NPs was active against 7 bacterial isolates (3 clinical and 4 environmental), and 1000µg/ml concentration of TiO2 NPs was effective against 9 isolates (6 clinical and 3 environmental ). These concentrations were mixed with the antibiotics Levloxacin LEV and Amikacin AK to investigate the possibility of synergistic activity against studied bacteria. Bacterial isolate's response or sensitivity to the antibiotic and TiO2 NPs mixture was varied; AK plus 500µg/ml TiO2 NPs concentration showed increased inhibitory activity against 7 isolates (3 clinical, 4 environmental) and 1000µg/ml TiO2 NPs mixed with AK showed increased inhibition activity against one environmental bacterial isolates, where Ak mixed with 500 and Ak plus 1000 µg/ml showed the same effect as the antibiotic alone or less.LEV antibiotic shows no difference in the effect on all 9 bacteria (7 clinical and 2 environmental), while LEV mixed with 500 µg/ml have increased inhibition zones on 4 bacteria (2 clinical, 2 environmental) , and LEV mixed with 1000µg/ml have higher effect than the antibiotic alone on three isolates (2 clinical,1 environmental). Keywords: antibiotic; titanium nanoparticles dioxide; antibacterial.