Abstract
The double staining procedure using both the nitroblue tetrazolium (NBT) and copper ferrocyanide methods was presented for the simultaneous localization of two different dehydrogenase activities in a single histologic section.Sections of normal mouse liver were stained for alcohol dehydrogenase (ADH) activity using the NBT method first, then stained for aldehyde dehydrogenase (ALDH) activity using the copper ferrocyanide method. However, the formazan showing ADH activity must chelate metal ion (Cu2+, Co2+, or Ni2+) prior to staining for ALDH activity.Both the blue metal-formazan complex showing ADH and brown copper ferrocyanide showing ALDH activities in a single liver section were localized mainly in the perivenous zone of the hepatic lobule. In addition, ALDH activity is always localized in the zone of the lobule where ADH activity is present.
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