Double imprinting in a single molecularly imprinted polymer format for the determination of ascorbic acid and dopamine

Mahavir Prasad Tiwari,Darshika Jauhari,Bhim Bali Prasad,Rashmi Madhuri,Deepak Kumar

doi:10.5185/amlett.indias.202

Abstract

A new molecularly imprinted polymer – carbon composite fiber is constructed using reversible addition-fragmentation chain transfer polymerization technique. The fiber was evaluated as a sensor for the simultaneous determination of ascorbic acid and dopamine at ultratrace level, in aqueous samples, without any cross-reactivity. The binding characteristics of ascorbic acid and dopamine were also evaluated by differential pulse cathodic stripping voltammetry.

Highlights

Artificial molecular recognition elements prepared by imprinting techniques attract increasing attention due to their potentials to act as selective sorbents in analytical sample pretreatment, chemical sensing, drug delivery, catalysis, dedicated separations, and screening elements in drug discovery
Unlike the general molecularly imprinted polymer (MIP)-composite electrode preparation protocol [3], we have resorted to a fresh approach for an in-situ synthesis of MIPcarbon composite fiber via reversible additionfragmentation chain transfer (RAFT) polymerization technique
The proposed MIP-carbon composite electrode was examined for the simultaneous determination of ascorbic acid (AA) and dopamine (DA), in aqueous samples using differential pulse cathodic stripping voltammetry (DPCSV)

Summary

Introduction

Artificial molecular recognition elements prepared by imprinting techniques attract increasing attention due to their potentials to act as selective sorbents in analytical sample pretreatment, chemical sensing, drug delivery, catalysis, dedicated separations, and screening elements in drug discovery. In most of the accounts of molecular imprinting, a single template molecule is used to create specific binding sites in the molecularly imprinted polymer (MIP) motif. Unlike the general MIP-composite electrode preparation protocol [3], we have resorted to a fresh approach for an in-situ synthesis of MIPcarbon composite fiber via reversible additionfragmentation chain transfer (RAFT) polymerization technique. RAFT is a degenerative chain transfer free radical polymerization process Such living radical polymerization technique is reportedly known to be creating homogeneous binding sites (cavities) within the MIP network. The proposed MIP-carbon composite electrode was examined for the simultaneous determination of ascorbic acid (AA) and dopamine (DA), in aqueous samples using differential pulse cathodic stripping voltammetry (DPCSV). The present work deals, for the first time, with the high selectivity and sensitive simultaneous determination of AA and DA

Materials and reagents

Sensor fabrication and voltammetric procedure

Spectral characterization and recognition mechanism

Simultaneous determination of AA and DA in aqueous medium

Conclusion

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