Abstract
Bateriophage (Φ29, SPP1, or SPO1)-infected, toluene-treated minicells of Bacillus subtilis are capable of limited amounts of non-replicative DNA synthesis as measured by incorporation of [ 3H]dTTP into a trichloroacetic acid-precipitable form. The [ 3H]dTTP is covalently incorporated into small DNA fragments which result from the degradation of a small percentage of the infecting phage genomes (molecular weights in the range of 2 · 10 5). Short exposure of the DNA molecules containing the incorporated [ 3H]dTMP to Escherichia coli exonuclease III results in over 90% of the [ 3H]dTMP being converted to a trichloroacetic acid-soluble form. The synthesis is totally dependent on host-cell enzymes and is not inhibited by the addition of chloramphenicol, rifampicin, nalidixic acid and mitomycin C and only slightly (approx. 20%) inhibited by the addition of 6-(p- hydroxyphenylazo)-uracil .
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