Abstract
1. 1. Chick-liver mitochondria incorporate deoxyribonucleosides and dNTP's into endogenous mitochondrial DNA. The maximal rate of dATP incorporation was 0.5 pmole/mg protein per 10 min. This corresponds to about 0.1 % net synthesis. The apparent K m for dATP in the presence of 15 μM of the other dNTP's was 1.5 · 10 −6 M. 2. 2. dATP incorporation into acid-insoluble material by isolated chick-liver mitochondria was not inhibited by phenethyl alcohol and only to a limited extent by high concentrations of nalidixic acid. Half-maximal inhibition by ethidium bromide was observed at about 1 μM and 12.5 μM was sufficient to completely block the incorporation of dATP in high-molecular-weight DNA. 3. 3. Up to 70 % of the purified product DNA co-sedimented with closed circular duplex mitochondrial DNA in sucrose gradients and CsCl gradients containing ethidium. Treatment of this I∗ with deoxyribonuclease converted it into material with the sedimentation properties of open circular mitochondrial DNA (II∗).
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