Abstract
Abstract: It has recently become possible to synthesize in vitro double stranded DNA from specific mammalian mRNA templates and to introduce these gene sequences into bacterial plasmids(I). Such plasmids have been constructed containing α and β globin gene sequences from rabbit and mouse and immunoglobulin light chain sequences from mouse. These plasmids are now used for the purification of the mRNA and cDNA probes necessary in the study of immunoglobulin genes. They have also been used for the simultaneous purification of the + and the − strands of cellular DNA restriction fragments carrying the light chain genes.
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