Distribution of peripheral blood lymphocyte populations in patients with primary Sj�gren�s syndrome

Abstract

Event Abstract Back to Event Distribution of peripheral blood lymphocyte populations in patients with primary Sjögren’s syndrome Gintaras Sudzius1*, Dainius Characiejus1, Diana Mieliauskaite2, Almantas Siaurys1, Irena Butrimiene2 and Irena Dumalakiene1 1 State Research Institute Center for Innovative Medicine, Department of Immunology, Lithuania 2 State Research Institute Center for Innovative Medicine, Department of Innovative diagnostic treatment and health monitoring technology, Lithuania Distribution of lymphocyte populations’ in peripheral blood mononuclear cells (PBMCs) of patients with primary Sjögren's syndrome (pSS) without (1pSS) or with (2pSS) extraglandular manifestations were investigated. 30 1pSS, 23 2pSS and 35 healthy controls were enrolled in the study. PBMCs were stimulated in whole blood with PMA and ionomycin, PBMCs labelled for CD3, CD4, CD8, CD19, CD16+56, CD25, CD27, CD57, Foxp3 and IL-17A and analyzed by flow cytometry. Decline in CD3+ T cells in PBMCs of patients with pSS was detected. The reason for this was significantly decreased absolute counts of CD4+, CD8+ and CD3+CD16+56 cells in patients with pSS. Significant decrease in absolute counts of CD4+IL17A+ and CD8brightCD57-CD27+ cells in patients with pSS were observed as compared to control group. Absolute counts of CD4+25brightFoxp3 and CD19+ cells were similar in all three groups and CD3-CD16+56 cells was significantly decreased only in 1pSS group. All results between 1pSS and 2pSS groups were comparable. However, different correlations between lymphocyte populations in 1pSS and 2pSS groups were found. Only in 1pSS the correlation between CD3-CD16+56 and CD3+CD16+56, CD3-CD16+56 and CD4+25brightFoxp3, CD4+ and CD19+, CD4+ and CD8brightCD57-CD27+ cells was registered. Whereas, in 2pSS the correlation between CD4+ and CD4+IL-17A+, CD8+ and CD3+CD16+56, CD4+IL-17A+ and CD8brightCD57-CD27+ cells was revealed. Declining and variations of T-lymphocyte subsets in pSS patients` could be the result of increased apoptosis and/or migration to inflamed tissues of CD4+IL-17A+, CD8brightCD57-CD27+, CD3+CD16+56 and CD3-CD16+56 (only in 1pSS group) cells. We suppose that these cell populations play an important role in pSS pathogenesis. Acknowledgements This research was funded by a grant No. LIG-23/2010 from the Research Council of Lithuania. Keywords: primary Sjögren's syndrome, extraglandular manifestations, distribution of lymphocyte populations, PBMCs, lymphocitopenia Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Sudzius G, Characiejus D, Mieliauskaite D, Siaurys A, Butrimiene I and Dumalakiene I (2013). Distribution of peripheral blood lymphocyte populations in patients with primary Sjögren’s syndrome. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00226 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Gintaras Sudzius, State Research Institute Center for Innovative Medicine, Department of Immunology, Vilnius, Lithuania, gintaras.sudzius@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gintaras Sudzius Dainius Characiejus Diana Mieliauskaite Almantas Siaurys Irena Butrimiene Irena Dumalakiene Google Gintaras Sudzius Dainius Characiejus Diana Mieliauskaite Almantas Siaurys Irena Butrimiene Irena Dumalakiene Google Scholar Gintaras Sudzius Dainius Characiejus Diana Mieliauskaite Almantas Siaurys Irena Butrimiene Irena Dumalakiene PubMed Gintaras Sudzius Dainius Characiejus Diana Mieliauskaite Almantas Siaurys Irena Butrimiene Irena Dumalakiene Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.