The characteristic of minor cell populations in multiple sclerosis patients after cell therapy

Abstract

Event Abstract Back to Event The characteristic of minor cell populations in multiple sclerosis patients after cell therapy Darya Nizheharodava1*, Alexay Borisov2, Ekatherina Nazarova3, Ekatherina Rudakovskaya1 and Maryna Zafranskaya1 1 Belarussian Medical Academy of Post-Graduated Education, Immunology Department, Belarus 2 Belarusian State Medical University, Neurology Department, Belarus 3 Minsk City Hospital N9, Transplantology Department, Belarus Introduction. Minor cell populations demonstrate functional diversity, from effector action to immunoregulation, depending on pathological conditions. Our previous results showed significant differences in numbers and functions of minor cell populations including γδT-cells, СD8lowT-cells, NK-cells and TNK-cells in multiple sclerosis (MS) patients compared with donors. Objective. To estimate the influence of cell therapy upon minor cell populations’ quantity in peripheral blood of MS patients. Materials and methods. Peripheral blood mononuclear cells (PBMC) were obtained from relapse-remitting MS patients (n=15) with expanded disability status scale (EDSS) score median of 2,5 before and after intravenous infusion of autologous bone marrow-derived mesenchymal stem cells. PBMC were harvested at baseline and during 1 year after cell therapy and monitored with flow cytometer FC500 using appropriate antibodies. Results. γδT-cells, which are able to regulate myelin-specific T-cell response, increased in peripheral blood of MS patients on day 10 after cell therapy (p<0,01) and continued elevation during 1 year of treatment (p<0,05) without any significant changes in СD8+γδT-cells population with potential cytotoxic activity. The percent of СD8lowT-cells reduced after 1 month of cell infusion (p<0,01) and the decrease of СD8lowCD45RO+T-cells and СD8lowCD119+T-cells populations was observed during 9 months after treatment (p<0,01). The last one correlated with activated СD3+CD25+T-cells’ percent (R=0,59, p<0,05). The number of NK-cells correlated with EDSS before treatment (R=0,54, p<0,01) and decreased after 1 month of cell therapy (p<0,05) without significant differences in TNK-cells percentage. Conclusion. Cell therapy established immunoregulatory profile of minor cell populations in peripheral blood of MS patients following 1 year after treatment. Acknowledgements This work was supported by Ministry of Health Care of Republic of Belarus grants N20081618 and N20112544. Keywords: Multiple Sclerosis, cell therapy, minor cell populations, myelin-specific response, Mesenchymal Stem Cells Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Nizheharodava D, Borisov A, Nazarova E, Rudakovskaya E and Zafranskaya M (2013). The characteristic of minor cell populations in multiple sclerosis patients after cell therapy. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00590 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 May 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Darya Nizheharodava, Belarussian Medical Academy of Post-Graduated Education, Immunology Department, Minsk, Belarus, nzh@tut.by Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Darya Nizheharodava Alexay Borisov Ekatherina Nazarova Ekatherina Rudakovskaya Maryna Zafranskaya Google Darya Nizheharodava Alexay Borisov Ekatherina Nazarova Ekatherina Rudakovskaya Maryna Zafranskaya Google Scholar Darya Nizheharodava Alexay Borisov Ekatherina Nazarova Ekatherina Rudakovskaya Maryna Zafranskaya PubMed Darya Nizheharodava Alexay Borisov Ekatherina Nazarova Ekatherina Rudakovskaya Maryna Zafranskaya Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.