The cytokine production of peripheral blood mononuclear cells reflects the autoantibody profile of patients suffering from type 1 diabetes

Abstract

Event Abstract Back to Event The cytokine production of peripheral blood mononuclear cells reflects the autoantibody profile of patients suffering from type 1 diabetes Jana Labiková1*, Jana Včeláková1, Stanislava Koloušková1 and Kateřina Štechová1 1 Peadiatric Dpt. University Hospital Motol 2nd Medical Faculty, Charles University, Czechia Type 1 diabetes (T1D) is an autoimmune disease characterised by the destruction of pancreatic beta-cells. Proinflammatory Th1 response is considered as the most pathological. Specific autoantibodies can be detected a long time before the disease clinical onset. This study describes the influence of synthetic diabetogenic autoantigens GAD65 and IA2 on the cytokine response of peripheral blood mononuclear cells (PBMC) obtained from T1D patients with regards to their antibody profile. The study has been carried out on T1D patients (n = 40) positive for anti-GAD65 and/or anti-IA2 autoantibodies. Using flow cytometry we measured the cell type ratio in PBMC samples. The cells have been stimulated by three different concentrations of mentioned antigens and their IFN-gamma and IL-17 production has been detected by ELISPOT assay. In the case of both cytokines we have found a significant interaction between the way of stimulation (type and antigen concentration) and the patient's antibody profile (p = 0.003 IFN-gamma; p < 0.001 IL-17). The IA2 autoantibodies level correlates positively with the proportion of Tc cells and negatively with the proportion of Th cells (p = 0.004; p = 0.001). The IFN-gamma production correlates positively with the proportion of T cells and negatively with the proportion of NK cells in PBMC (p = 0.001; p = 0.001). Our results show that patient´s autoantibody profile reflects the type of his/her cellular responses too. This approach can be helpful in designing immunointervention strategies aimed at preventing T1D onset. Such immunointevention should apparently be individualised. Acknowledgements The study was supported by the project (Ministry of Health, Czech Republic) for conceptual development of research organization 00064203 (University Hospital Motol, Prague,Czech Republic), IPL699001 and IGA5240 Keywords: type 1 diabetes, PBMC, GAD65, IA2, IFN-gamma Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Labiková J, Včeláková J, Koloušková S and Štechová K (2013). The cytokine production of peripheral blood mononuclear cells reflects the autoantibody profile of patients suffering from type 1 diabetes. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00483 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Jana Labiková, Peadiatric Dpt. University Hospital Motol 2nd Medical Faculty, Charles University, Prague, Czechia, jana.labik@seznam.cz Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jana Labiková Jana Včeláková Stanislava Koloušková Kateřina Štechová Google Jana Labiková Jana Včeláková Stanislava Koloušková Kateřina Štechová Google Scholar Jana Labiková Jana Včeláková Stanislava Koloušková Kateřina Štechová PubMed Jana Labiková Jana Včeláková Stanislava Koloušková Kateřina Štechová Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.