Abstract
The distribution and some properties of DNA polymerase in purified subcellular fractions from rat intestinal mucosa were studied. Activity was found in Tris-and NaCl-soluble extracts of nuclei, in association with mitochondria, ribosomes and smooth membranes, and also in a soluble cytoplasmic fraction. Evidence of only three distinct polymerases was obtained when the activities from these fractions were compared on the basis of their pH optimum, Mg 2+ requirement and their affinity for DEAE-cellulose. In order of increasing anionic charge, the first accounted for the principal activity in the Tris-soluble nuclear, ribosomal and soluble cytoplasmic fractions, the second constituted the major activity in the NaCl-soluble nuclear fraction and the third was associated with mitochondria and smooth membranes.
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