Abstract
Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-l-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03.
Highlights
Many bacteria in association with plants or animals utilize cell-to-cell communication to monitor their virulence determinants [1]
Two major Quorum sensing (QS) system signaling molecules namely autoinducer-1 N-acyl homoserine lactone (AHL)-based system commonly used by most Gram-negative bacteria and autoinducer-2, oligopeptide-based system used by most Gram-positive bacteria [6,7]
We investigated the presence of QS bacteria in a Malaysian tropical rainforest waterfall sample, and report the isolation of a QS bacterium, Pantoea rodasii ND03
Summary
Many bacteria in association with plants or animals utilize cell-to-cell communication to monitor their virulence determinants [1]. The species clustered in the genus Pantoea have been documented to show QS properties, but their possible association with hosts and disease remain unclear, for example, the role of QS in Pantoea stewartii that infects corn [11]. The pathogenic effect is believed to be influenced by regulation of virulence determinants throughout the infection process and QS allows these pathogenic bacteria to make a concerted attack and produce ample virulence factors to overwhelm the host defenses [13]. This triggered interest in our group to extend this research in isolating environmental water-borne bacteria that possess QS properties. We investigated the presence of QS bacteria in a Malaysian tropical rainforest waterfall sample, and report the isolation of a QS bacterium, Pantoea rodasii ND03
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