Abstract
Direct tissue isoelectric focusing (DTIF) of 4-70 μm sections of frozen autopsy material from central nervous system (CNS) and peripheral nervous system (PNS), and from muscle biopsy, followed by immunofixation with unlabelled or radiolabelled antiserum against human γ chains or IgG Fc fragments has been developed for analysis of IgG patterns. In multiple sclerosis (MS) brains, oligoclonal IgG bands were found which differed regarding number and mobility between the brains, and also between different regions in individual brains. The DTIF technique followed by antiserum immunofixation may be a powerful tool for protein analysis in autopsy and biopsy specimens from the nervous and muscular systems.
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