Abstract
A simple, direct and cost-effective spectrophotometric method for determination of cefuroxime axetil (CRXA) in pure and tablet dosage forms was applied. This method is based on formation of ion-pair complex ([CRXA]:[BTB]) between CRXA and bromothymol blue (BTB) in chloroform. Beer’s law in the optimum experimental conditions using [CRXA]:[BTB] complex is valid within a concentration range of 2.00-50.00 ?M (1.021–25.524 ?g.mL-1). The developed method is applied for the determination of CRXA in pure and its commercial tablets without any interference from excipients with average assay of 96.8 to 101.6% and the results are in good agreement with those obtained by the HPLC reference method. Associated drugs (sulbactam and linesolid) with cefuroxime axetil are considered to be interfere, while metronidazole can be considered as non-interfere.
Highlights
Cefuroxime axetil (CRXA) is (RS)-1-hydroxyethyl (6R,7R)-7-[2-(2-furyl) glyoxyl-amido]-3(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]-oct-2-ene-2-carboxylate,72-(Z)-(O-methyl-oxime), 1-acetate 3-carbamate], its trade name is Ceftin, mol. mass of cefuroxime axetil (CRXA) is 510.475 g/mol and 424.38 g/mol for cefuroxime (CRX), see Scheme 1 [1]
Cefuroxime axetil (CRXA) is (RS)-1-hydroxyethyl (6R,7R)-7-[2-(2-furyl) glyoxyl-amido]-3(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]-oct-2-ene-2-carboxylate,72-(Z)-(O-methyl-oxime), 1-acetate 3-carbamate], its trade name is Ceftin, mol. mass of CRXA is 510.475 g/mol and 424.38 g/mol for cefuroxime (CRX), see Scheme 1 [1]. It is a broad spectrum, semi synthetic cephalosporin antibiotics which is administered for treating variety of bacterial infections such as case of pharyngitis, chronic bronchitis and infection of urinary tract as well as uncomplicated
3.3 Optimization of Variables The different experimental parameters affecting the spectrophotometric determination of CRXA through ionpair complex [CRXA]:[bromothymol blue (BTB)] formation with BTB in chloroform was studied in order to determine the optimal conditions for the determination of CRXA. 3.4 The effect of time and temperature The effect of time and temperature on the complex [CRXA]:[BTB] formation was studied within the ranges 5120 min and 15-45oC
Summary
Cefuroxime axetil (CRXA) is (RS)-1-hydroxyethyl (6R,7R)-7-[2-(2-furyl) glyoxyl-amido]-3(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]-oct-2-ene-2-carboxylate,72-(Z)-(O-methyl-oxime), 1-acetate 3-carbamate], its trade name is Ceftin, mol. mass of CRXA is 510.475 g/mol and 424.38 g/mol for cefuroxime (CRX), see Scheme 1 [1]. Mass of CRXA is 510.475 g/mol and 424.38 g/mol for cefuroxime (CRX), see Scheme 1 [1] It is a broad spectrum, semi synthetic cephalosporin antibiotics which is administered for treating variety of bacterial infections such as case of pharyngitis, chronic bronchitis and infection of urinary tract as well as uncomplicated. It is a second generation cephalosporin and can be administered orally as well as in the parenteral dosage form. Bromothymol blue C27H28Br2O5S (BTB), acts as a weak acid in solution It can be in protonated or deprotonated form, appearing yellow or blue, respectively. Bromothymol blue has been used as a reagent to form ion pair complexes with drugs [4]
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