Spectrophotometric Determination of Gemifloxacin Mesylate, Moxifloxacin Hydrochloride, and Enrofloxacin in Pharmaceutical Formulations Using Acid Dyes

Amira G Yousef,Ayman A Gouda,Ragaa El-Sheikh,Alaa S Amin

doi:10.1155/2014/286379

Amira G Yousef, Ayman A Gouda + Show 2 more

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https://doi.org/10.1155/2014/286379

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Abstract

Simple, rapid, and extractive spectrophotometric methods were developed for the determination of some fluoroquinolones antibiotics: gemifloxacin mesylate (GMF), moxifloxacin hydrochloride (MXF), and enrofloxacin (ENF) in pure forms and pharmaceutical formulations. These methods are based on the formation of ion-pair complexes between the basic drugs and acid dyes, namely, bromocresol green (BCG), bromocresol purple (BCP), bromophenol blue (BPB), bromothymol blue (BTB), and methyl orange (MO) in acidic buffer solutions. The formed complexes were extracted with chloroform and measured at 420, 408, 416, 415, and 422 nm for BCG, BCP, BPB, BTB, and MO, respectively, for GMF; at 410, 415, 416, and 420 nm for BCP, BTB, BPB, and MO, respectively, for MXF; and at 419 and 414 nm for BCG and BTB, respectively, in case of ENF. The analytical parameters and their effects are investigated. Beer's law was obeyed in the ranges 1.0–30, 1.0–20, and 2.0–24 μg mL−1 for GMF, MXF, and ENF, respectively. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations. Statistical comparison of the results with the reference methods showed excellent agreement and indicated no significant difference in accuracy and precision.

Highlights

Fluoroquinolones are the second-generation members of quinolone antibiotics fluorinated in position 6 and bearing a piperazinyl moiety at position
We report the development of accurate and precise extractive spectrophotometric methods based on the chloroform soluble ion-pair complexes between the studied fluoroquinolone antibiotics (GMF, MXF, and ENF) and some acid dyes (BCG, bromocresol purple (BCP), bromothymol blue (BTB), bromophenol blue (BPB), or methyl orange (MO))
This paper describes the application of extractive ion-pair complexation reaction with acid dyes for the quantification of some fluoroquinolones antibiotics (GMF, MXF, and ENF) in pure forms and pharmaceutical formulations

Summary

Introduction

Fluoroquinolones are the second-generation members of quinolone antibiotics fluorinated in position 6 and bearing a piperazinyl moiety at position. They are considered to be the most effective Gram-positive and Gram-negative pathogens to combat infection caused by microorganisms that are resistant to other microbials, such as tetracyclines. Moxifloxacin (MXF) is {1-cyclopropyl-7-[2,8-diazobicyclo (4.3.0) nonane]-6-fluoro-8-methoxy-1,4 dihydro-4-oxo-3-quinolone carboxylic acid}. Enrofloxacin (ENF) is (1 cyclopropyl-7-(4-ethyl-1-piperazinyl)6-fluoro-1,4-dihydro-4-oxo-3-quinolone carboxylic acid) (Scheme 1). GMF and MXF are fourth-generation synthetic broad-spectrum 8-methoxy fluoroquinolone antibacterial drug derivatives. Due to their clinical advantages, GMF and MXF are receiving a great interest and there was an increase in number of their pharmaceutical dosage forms in the market in the recent past. ENF is official in the United States Pharmacopeia (USP) [4]

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