Abstract
Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively. First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB). The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.
Highlights
(GB) is 1-[4-[2-[(5-Chloro-2-methoxybenzoyl)amino]ethyl]phenyl]sulphonyl]-3-cyclohexylurea, is oral hypoglycemic drug sulphonyl ureas second generation act by inhibiting ATP-sensitive potassium channels in pancreatic beta cells
Stock solution 4.05 × 10-3 M of (GB), MW = 494 g/mol, was prepared by dissolving 40 mg of (GB) in Potassium hydroxide 0.1 M, equivalent to 40.04 mg by taken the purity in consideration in volumetric flask 20 mL.The working standard solutions for zero spectrophotometric method were prepared by appropriate dilutions of stock solution 4.05 × 10-3 M in KOH 0.1 M to give concentrations between (4 360) μg/mL of (GB).The working standard solutions for derivative spectrophotometric method were https://rajpub.com/index.php/jac prepared by appropriate dilutions of stock solution 4.05 × 10-3 M in KOH 0.1 M to give concentrations between (4 - 240) μg/mL of (GB)
Zero spectrophotometry: Absorption spectra of the standard raw material samples 40 μg/mL (GB) and 15 μg/mL (MET) solutions were recorded within a wavelength range of (225 – 350) nm against the blank 0.1 M of KOH (all the addition constituents without (GB) and
Summary
(GB) is 1-[4-[2-[(5-Chloro-2-methoxybenzoyl)amino]ethyl]phenyl]sulphonyl]-3-cyclohexylurea, is oral hypoglycemic drug sulphonyl ureas second generation act by inhibiting ATP-sensitive potassium channels in pancreatic beta cells This inhibition causes cell membrane depolarization, which cause voltage dependent calcium channels to open, which causes an increase in intracellular calcium in the beta cell, which stimulates insulin release[1,2]. The estimation of (GB) from pharmaceutical formulations has been determining by several analytical methods These include UV spectroscopy methods[3,4,5,6,7,8,9], spectrofluorimetric method[10], reversed-phase high-performance liquid chromatography (RP-HPLC) methods[11,12] and high performance liquid chromatography (HPLC)[13,14], high performance thin layer chromatography (HPTLC)[15,16]
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