Abstract
Candidemia and invasive candidiasis is a cause of high mortality and morbidity rates among hospitalized patients worldwide. The occurrence of the infections increases due to the complexity of the patients and overuse of the antifungal therapy. The current Candida detection method includes blood culturing which is a lengthy procedure and thus delays the administration of the antifungal therapy. Even though the results are available after 48 h it is still the gold standard in pathogen detection in a hospital setting. In this work we present an electrochemical impedance sensor that is capable of detecting Candida albicans yeast. The yeast cells are captured on electrodes specifically functionalized with anti-Candida antibodies and detection is achieved by electrochemical impedance spectroscopy. The sensor allows for detection of the yeast cells at clinically relevant concentrations in less than 1 h.
Highlights
Patients with impaired immunoprotection have a high risk of infection
We present a membrane-based electrochemical sensor with a functional layer of anti-C. albicans antibodies able to detect the presence of C. albicans spiked in PBS at a concentration as low as
The anti-Candida antibodies used here were modified with the LC-SPDP molecule, which is a linker molecule that introduces disulfides to primary amines on antibodies
Summary
Patients with impaired immunoprotection have a high risk of infection. The risk group is incredibly large, including cancer, HIV positive or post-transplant patients [1,2]. There are many pathogenic microorganisms such as virus, bacteria or fungi that can cause a disease in a population of patients with an impaired immune response. Candidiasis is mainly an endogenous infection produced by the overgrowth of fungi due to physiological changes in the body, e.g., the imbalances in the gut microbiota [3,6,7]. It can be acquired from sources like catheters or prosthetic devices, by person-to-person or vertical transmission [1,3,6,8,9]. Invasive candidiasis is the fourth most common cause of hospital-acquired infections and Candida sp. are responsible for 50%
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
