Abstract
Background The present study is aimed at providing systematic insight into the composition and expression of transfer RNA (tRNA) derivative transcription in high-grade serous ovarian cancer (HGSOC). Methods tRNA derivative expression profiles in three pairs of HGSOC and adjacent normal ovarian tissues were conducted by tRNA-derived small RNA fragment (tRF) and tRNA half (tiRNA) sequencing. The differentially expressed tRFs and tiRNAs between HGSOC and paired adjacent normal samples were screened. The targeted genes of differentially expressed tRFs and tiRNAs were screened. The Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) of target genes of tRFs and tiRNAs were analyzed. Results There are a total of 20 significantly upregulated and 15 significantly downregulated tRFs and tiRNAs between the cancer group and the paracarcinoma group. The upregulated tRFs and tiRNAs are mucin-type O-glycan biosynthesis, glycosphingolipid biosynthesis, the glucagon signaling pathway, the AMPK signaling pathway, maturity-onset diabetes of the young, glycosphingolipid biosynthesis, the insulin signaling pathway, insulin resistance, leukocyte transendothelial migration, starch, and sucrose metabolism. The downregulated tRFs and tiRNAs are other glycan degradation, vitamin digestion and absorption, fatty acid elongation, and biosynthesis of unsaturated fatty acids. Conclusions There are significantly expressed tRFs and tiRNAs in HGSOC tissues, and these may provide potential diagnostic biomarkers and therapeutic targets for HGSOC.
Highlights
The present study is aimed at providing systematic insight into the composition and expression of transfer RNA derivative transcription in high-grade serous ovarian cancer (HGSOC)
TRNA-derived fragments and transfer RNA (tRNA) halves are small noncoding RNAs derived from precursor tRNAs or mature tRNAs [4]
Transfer RNA-derived fragments, 14–35 nucleotides, are a novel class of noncoding RNA rooted in tRNAs [6,7,8]. tRNA-derived small RNA fragment (tRF) were classified into tRF-5, tRF-3, and tRF-1 in prostate cancer [9]. tRFs play pivotal roles in cell
Summary
Ovarian cancer (OC) is one of the deadliest gynecological malignancies in the world [1, 2]. TRNA-derived fragments (tRFs) and tRNA halves (tiRNAs) are small noncoding RNAs derived from precursor tRNAs or mature tRNAs [4]. There were significantly expressed tRNA-derived fragments in breast cancer tissues, which are hopefully to become biomarkers and valuable researches in this area [15]. The expression profile of tRNA-derived fragments was analyzed in pancreatic cancer [17]. Read in conjunction, these researches showed a functional role of tRNA derivatives in tumorigenesis. We screened tRNA derivative profiles of HGSOC patients using high-throughput tRF and tiRNA sequencing.
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