Different colony stimulating factor on the effect of acute myeloid leukemia in patients with complete remission of dendritic cell subsets

Abstract

Objective To study the granulocyte colony stimulating factor（G-CSF） and granulocyte macro- phage colony stimulating factor（GM-CSF） in complete remission of acute myeloid leukemia（AML） patients with den- dritic cells （DC） changes in the function and its subsets. Methods 36 cases of complete remission according to AML patients were randomly divided into 3 groups, G group was given G-CSF 200g/d, subcutaneous injection, GM group were given GM-CSF 200g/d, subcutaneous injection, the control group were injected with normal saline. The curative effects were compared. Results The expression of G in group DC, CD83 and CD86 on surface of CD80 were lower than that in GM group and C group （ t = 4.34,5.43,4.54,4.54,5.25,3.54, all P 〈 0. 05 ）, GM group, DC expression of CD11 c was higher than that of G group and C group （t = 4.54,4.27, all P 〈 0.05 ）. G group and GM group DC in promoting lymphocyte proliferation were higher than those in C group （ t = 4.54,5.64, all P 〈 0.05 ） ; group GM DC to promote the ability of CD4 ~ T lymphocyte proliferation was higher than that of G group （ t -- 3.54, P 〈 0.05 ）. ELISA assay,GM group DC secretion of IL-12 than that of group G and C group;group G DC secretion of IL-10 was higher than that of GM group and C group （ t = 3.54.4.23,4.32,3.87, all P 〈 0.05 ）. Conclusion G-CSF and GM-CSF can make the complete remission in patients with AML subgroup DC shift, the bias of G-CSF DC2 and certain immuno- suppressive effect,while GM-CSF DC subsets of DC1 and promotes cell immune deviation. Key words: Colony-stimulating factors; T-lymphocyte subsets ; Leukemia, myeloid