Abstract
The estimation of non‐volatile carboxylic acids in plant material by gas‐liquidchromatography..A method for the estimation of non volatile carboxylic acids in plant material by GLC is described. The organic acids or salts are extracted by 1 N HC1, separated from the cations, the neutral constituents, and the stronger mineral acids by ion exchangers. After the elutriation the acids are dried, dissolved in tetrahydrofurane, and transferred into trimethylsilyl derivatives by bis (trimethylsilyl) acetamide (BSA).The retention time and the specific peak area of 19 carboxylic acids are given. The reliability of the data is tested in three ways by the estimation of the total variance, the calculation of the variances of the different analytical steps by the aid of the variance analysis, and the determination of the losses. Malonate, succinate, fumarate, malate, aconitate, citrate, shikimate, and quinate are quantitatively determinable. In addition it seems feasible to estimate tartrate, cis and trans aconitate, citrate and isocitrate if both isomeric compounds in the same plant sample are present by the use of several different columns and to estimate uronic acids by the application of another silylating reagent. Oxalate and glycerate are indeterminable with this method.The time needed for 1 analysis is less than 1 hour. As the extraction and the chromatographic seperation should be repeated it is practicable to analyse the average of 2 plant samples a day.
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