Abstract
Na-K-ATPase is intimately associated with the active transport of electrolytes in many tissues. In rabbit nephrone segment, dexamethasone has been proved to stimulate the Na-K-ATPase activity. Here we studied the influence of dexamethasone on Na-K-ATPase activity of rabbit iris-ciliary body. The iris-ciliary body of albino rabbit was placed in RPMI medium supplimented with 10% fetal calf serum and dexamethasone. After 24 hours incubation, the Na-K-ATPase activity was measured in crude tissue homogenate. The baseline Na-K-ATPase activity was 401±37 nmoles Pi/mg protein/hr. Stimulation of Na-K-ATPase activity was observed over a range of dexamethasone concentration (0.01 to 1 μg/ml). Maximal stimulation (44% above control level) was achieved with 1 μg/ml dexamethasone. However at high concentrations (＞10 μg/ml), Na-K-ATPase activity was decreased markedly. These studies show that dexamethasone can stimulate Na-K-ATPase activity of rabbit irisciliary body at very low concentration, and this enzyme stimulation might have some contribution to the pressure elevation after dexamethasone administration in vivo.
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