Effect of autologous platelet-rich plasma on directional induced differentiation of rabbit bone marrow mesenchymal stem cells

Abstract

Objective To study the cell morphology and differentiation efficiency when rabbit bone marrow mesenchymal stem cells (BMSCs) were induced osteogenic differentiation as culturing by autologous platelet-rich plasma (PRP) instead of serum, and to explore a new method of inducing BMSCs osteogenic differentiation. Methods The PRP was prepared by arterial blood of rabbit. Punctured and The bone marrow was sampled from rabbit＇s iliac bone, and BMSCs were collected, which divided into PRP group, fetal calf serum (FBS) group and serum-free control group, and cultured in 10% autologous PRP, 10% FBS and serum-free respectively, combined with DMEM-F12 medium. The second generation cells were divided into experimental and control groups. The experimental groups＇ medium was added dexamethasone, β-sodium glycerophosphate and ascorbic acid, and the control groups went on. The cell morphological difference of each group was Observed between anterior and after inducing differentiation, and compared between each group. Results BMSCs of PRP and FBS groups grew quickly, presented like fusiform form before induction, and increasd in volume, became a triangle, polygonal and round form gradually after osteogenic induction. Cells of PRP and FBS groups aggregated spontaneously and multilayered, and formed calcium nodules and bone-like structure after induced 7 days averagely, which could be stained red by alizarin red S; cells of serum-free groups were induced 14 days averagely, only three samples showed osteogenesis performance. Cells of PRP and FBS groups differentiation efficiency was superior to serum-free groups when inducd 20 days, the difference was statistically significant (P 0.05). Conclusions Autologous PRP could be used to proliferate and induce osteogenic differentiation of BMSCs instead of serum. Key words: Rabbits; Bone marrow; Stromal cells; Stem cells; Platelet-rich plasma; Osteoblasts; Cell Differentiation