Development of Kopasanda Leaves Extract (Chromolaena odorata (L.)) Phytosome Using Solvent Evaporation Method.

Abstract

Kopasanda (Chromolaena odorata (L.) R.M.King &H.Rob) or siam weed exhibited various pharmacological activities such as anticancer, antidiabetic, and wound healing activities. The phenolic content of kopasanda leaf extract was high in the range of 56.15 mg GAE/g-242.3 mg GAE/g. But, it reported that phenolic compounds exhibited poor bioavailability because of their physicochemical properties. The phytosomes system improves the effectiveness of phytoconstituents. Aims of the research as prepare and evaluate the phytosome from kopasanda leaf extract. Kopasanda dried leaves were extracted by the sonication method. The solvent evaporation method was used in the phytosome preparation with various concentrations between extract and phospholipid ( 2:1; 1:1; 1:2; and 1:3). Furthermore, phytosome formation was analyzed for its entrapment efficiency, Polydispersity Index (PDI), particle size, spectroscopy infra-red (IR), and Transmission Electron Microscope (TEM) method. The total phenolic content (TPC) of crude was 110.969 ± 0.752 mgGAE/g. The optimum phytosome was gained with a concentration between extract and phospholipid (1:3). It resulted in the entrapment efficiency of 99.930 ± 0.001%, particle size, and polydispersity index equal to 206.5 ± 8.613nm and 0.528 ± 0.038. The morphology of the phytosome was spherical and the phytosome formation was verified by the IR spectrum by comparing the phytosome and the physical mixture of extract and phospholipid. The results obtained showed that phytosome containing kopasanda leaf extract was formed using the solvent evaporation method.