Abstract
The current study’s goal was to develop and validate an easy-to-use, highly-sensitive HPLC approach for simultaneously quantifying metformin and gliclazide in dosage forms on a single chromatographic run. Water symmetry Shield RP 8 column, 250 mm x 4.6 mm x 5 m, detection wavelength of 230 nm, mobile phase of buffer of pH 2.5 adjusted with orthophosporic acid and 1-mL triethylamine and methanol in a gradient programme for 25 minutes at 1.0-mL/min flow rate, 10 L injection volume, and 25 °C column temperature were the chromatographic conditions that were used. Retention times for metformin and gliclazide were found to be 2.23 and 14.88 minutes, respectively. According to ICH criteria, the method’s accuracy, precision, and resilience were evaluated.
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