Development and Validation of HPLC Method Using Hydrotropic Mobile Phase for the Estimation of Gatifloxacin

Patel Vandana B,Shah Dimal A,Maheshwari Rajesh K,Patel Matungi B

doi:10.36959/326/769

Abstract

In the present work eco-friendly, precise, accurate RP-HPLC method has been developed for the determination of gatifloxacin using the concept of hydrotropy. Hydrotropy is a concept of increasing solubility of a solute in water by adding an agent termed as hydrotropes. This concept reduces the use of organic solvents for analysis of drugs which makes analysis eco friendly and less expensive. The method was developed using 3% sodium benzoate (pH 6.5) as mobile phase and Shimadzu ODS-C18 column as stationary phase.

Highlights

Hydrotropy is a concept of increasing solubility of a solute in water by adding an agent termed as hydrotropes
Repeatability study was performed by analyzing target concentration six times over a short period of time which gave % RSD value less than 1% which indicates that the method is precise
Accurate reversed phase liquid chromatographic method has been developed for the estimation of Gatifloxacin from eye drop formulation

Summary

Introduction

Hydrotropy is a concept of increasing solubility of a solute in water by adding an agent termed as hydrotropes. Hydrotropes are amphiphilic substances composed of hydrophilic functional group used as a media for promoting solubilization of sparingly soluble substances in aqueous solution. Hydrotropes are organic salts which present in aqueous solutions can substantially improve the solubility of hydrophobic organic substances in the aqueous phase. Gatifloxacin (GATI) is chemically [1-cyclopropyl-6-fluoro-1, 4-dihydrogen-8-methoxy-7-(3-methyl-1-piperazinyl)-4-oxo-3quinoline] carboxylic acid (Figure 1). The molecular formula of GATI is C19H22FN3O4 and a molecular weight of 375.40 g/ mol [5]. It is antibacterial drug mainly used to treat bacterial conjunctivitis by inhibiting both bacterial DNA gyrase and topoisomerase IV [6-8]. DNA gyrase is an essential enzyme that is involved in the replication, transcription and repair of bacterial DNA

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Conclusion