New Analytical RP-HPLC Method Development and Validation for the Estimation of Prucalopride in Bulk and Pharmaceutical Dosage Form

Abstract

Background Prucalopride drug estimation was reported previously by UV spectroscopy methods and few HPLC methods with retention time 4.73 min as per literature survey. Aim of study A new analytical RP- HPLC method development and validation for bulk and pharmaceutical dosage forms of Prucalopride drug with less retention time than reported methods.Methodology The separation was carried out by using RP-HPLC with the mixture of 0.1 orthophosphoric acid methanol in ratio of 3070 vv as mobile phase. The mobile phase at a flow rate of 1 mLmin was pumped and UV detection was found at 225 nm.Results The selected chromatographic conditions gave retention time at 1.5 min for Prucalopride. Conclusion This developed method was validated for precision accuracy linearity robustness as per International Conference of Harmonization ICH guidelines.