Year-end Sale % OFF 
Abstract
In this present work, a simple, rapid and accurate HPLC-UV method has been developed for the quantification of andrographolide in rabbit plasma. The assay was performed using an XTerra® MS C18 column (150 mm X 4.6 mm, 5 μm) with a mobile phase of methanol and water (60:40), at 0.8 mL/min flow rate and UV detection of 229 nm. Andrographolide was extracted from a biological sample by applying acetonitrile as a precipitating and extraction solvent. The results showed a good linearity with r = 0.9992; the accuracy reported as % diff was found to be -6.42 – 6.55 % while the recovery was 99.09, 98.55, and 105.14% for low, medium and high spiked plasma, respectively. The precision (reproducibility) reached 1.08–3.20 % RSD for the sample studied. The 2.87 % relative standard deviation (RSD) value for selectivity test indicated a good selectivity of the developed method. The developed method is simple and rapid, so that it can be applied for the quantification andrographolide in animal models during pharmacokinetics studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
