Abstract
A method using high performance liquid chromatography with diode array detector (HPLC-DAD) for identification and quantification of pilocarpine in the extract of Pilocarpus microphyllus, popularly known as jaborandi. The analysis was conducted using RP-18 column (250 mm × 4.6 mm x 5 μm id) and a buffer solution composed of acidified water, phosphoric acid and triethylamine and methanol as a mobile phase in a flow rate of 1.0 mL/min and detection at 215 nm at 25 °C. Excellent linearity with r2 equal to 0.9999 was obtained. The recovery percentage was very satisfactory with values within the specifications. It is correct to affirm that the method has optimal intracurrent and intercurrent precision values with relative standard deviations of 0.1852% and 0.1932%, respectively. The robustness of the method, assessed through the Youden test, showed no significant influence of any of the evaluated parameters. In general, the method proved to be suitable for the intended purpose.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.