Abstract
Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) and high performance liquid chromatography with ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD and UPLC-UV methods were applied for the accurate determination of eleven major polyphenols in Moringa oleifera leaves. The chromatographic conditions of the eleven polyphenols was determined on two C18 column by gradient elution with 0.5 % phosphoric acid solution -acetonitrile as the eluate, and at a flow rate of 1.0 and 0.5 mL/min for HPLC-DAD and UPLC-UV methods, respectively. Detector parameter of UPLC-UV was fixed at 203 nm. The assay methods were validated systematically.Results: The instrumental methods (HPLC-DAD and UPLC-UV) had good linearity, precision,repeatability and recovery. For both methods, quantification limits of UPLC-UV (0.057 - 0.363 μg/mL) were lower than those of UPLC-UV (0.094 - 1.532 μg/mL). The UPLC method with a shorter running time and more sensitive detection was applied in comparing to the HPLC method. After optimization and evaluation, the baseline of 11 compounds was separated effectively within 68 and 34 min, respectively.Conclusion: The developed HPLC-DAD and UPLC-UV assays were successfully utilized for thesimultaneous analysis of eleven major polyphenols and can readily be utilized as quality control tools for Moringa oleifera leaves in China, with UPLC-UV method showing better separation, lower organic solvent usage and shorter analytical period.
Highlights
Moringa oleifera is a multipurpose plant native to India and widely distributed in many tropical and sub-tropical countries and regions [1]
Linearity was constructed on a series of 11 polyphenols standard solutions
LOD and LOQ of eleven polyphenols were determined by diluting the standard sample to a lower concentration
Summary
Moringa oleifera is a multipurpose plant native to India and widely distributed in many tropical and sub-tropical countries and regions [1]. A LC-MS method was exploited for the assay of twelve flavonoids constituents in the methanol fraction of leaf [14], while eleven phenolic compounds from Moringa oleifera leaves were determined by UPLC-ESI-MS/MS [15], the overhead costs of such equipment are higher. HPLC was successfully used for quantitative determination of three active compounds in Moringa oleifera leaves [16]. This method still has some defects including high organic solvent usage, long analytical period and poor resolution. Two chromatographic methods (HPLC-DAD and UPLC-UV) for effective components analysis in Moringa oleifera leaves. The two methods were established for determination the assay of 11 polyphenols in Moringa oleifera leaves from different habitats.
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