Development and Validation by UV Spectrophotometric method for Simultaneous Estimation of Fluconazole and Thymol in Formulation

Abstract

Abstract: Simultaneous equation technique has devised a simple, rapid, accurate, precise, and cost-effective spectrophotometric strategy for simultaneous estimation of Fluconazole and Thymol. Fluconazole and Thymol had absorbance maxima at 261 and 273 nm, respectively; hence absorbance was measured at these wavelengths for fluconazole and Thymol estimate. Fluconazole and Thymol followed Beer-Lambert's rule in concentration ranges of 20-140 g/ml and 20-100 g/ml, respectively. The technique was developed and verified in compliance with ICH guidelines and may be used to estimate Fluconazole and Thymol concentrations within formulations.