Ecofriendly Simple UV Spectrophotometric and Chemometric Methods for Simultaneous Estimation of Paracetamol Aceclofenac and Eperisone Hydrochloride in Pharmaceutical Formulation: Assessment of Greenness Profile

Seetharaman Rathinam,Lakshmi Karunanidhi Santhana

doi:10.3390/pr9081272

Seetharaman Rathinam, Lakshmi Karunanidhi Santhana

Open Access

https://doi.org/10.3390/pr9081272

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Journal: Processes	Publication Date: Jul 23, 2021
Citations: 3	License type: CC BY 4.0

Affiliation: SRM Institute of Science and Technology

Abstract

This work introduces three eco-friendly UV spectrophotometric methods for the simultaneous estimation of Paracetamol, Aceclofenac and Eperisone Hydrochloride in pharmaceutical tablet formulation. The procedures employed were simultaneous equation method and multivariate chemometric methods with phosphate buffer pH 7.80 as diluent. The simultaneous equation method encompasses absorbance measurement at three different wavelengths (λmax of the drugs). It exhibits linearity between 12–18 µg mL−1 for paracetamol, 3.69–5.53 µg mL−1 for Aceclofenac, and 2.76–4.15 µg mL−1 Eperisone hydrochloride. The results obtained for accuracy and precision by the simultaneous equation method were within the permissible limits. Principal component regression and partial least squares were the tools used for chemometric methods. The calibration set and prediction set were constructed, and the UV spectra were recorded in zero order mode, further subjected to chemometric analysis. The % recoveries obtained for Paracetamol, Aceclofenac, and Eperisone Hydrochloride by chemometric techniques showed good accuracy, and the results obtained for analytical figures of merit were acceptable. Statistical comparison of the assay results obtained for the proposed methods showed no significant difference found among the methods using one way analysis of variance. Greenness evaluation tools revealed the greenness profile of the proposed methods and found them to be ecofriendly. The described methods were appropriate for routine quality control laboratories, facilitating eco-friendly, fast, and cost effective determination of Paracetamol, Aceclofenac, and Eperisone Hydrochloride in Acemyoset P tablets.

Highlights

Paracetamol (PAR) (Figure 1a) is therapeutically used as an analgesic and antipyretic, while chemically, it is N-(-4-Hydroxy phenyl)-acetamide [1]
The developed spectrophotometric methods is preferable to highly sophisticated instruments like HPLC, HPTLC, and LCMS, which stipulate a tedious separation process but have the advantages of being eco-friendly, simple to use, fast, sensitive, and inexpensive
The solubility studies were made for the selected drugs in different buffers and solvents

Summary

Introduction

Paracetamol (PAR) (Figure 1a) is therapeutically used as an analgesic and antipyretic, while chemically, it is N-(-4-Hydroxy phenyl)-acetamide [1]. Aceclofenac (ACE) (Figure 1b) is chemically 2-[(2, 6 dichlorophenyl)-amino] benzene acetic acid carboxymethyl ester and used as an analgesic [1]. Eperisone Hydrochloride (ES) (Figure 1c) is therapeutically used as a muscle relaxant, and chemically, it is 1-(4-Ethylnylphenyl)-2-methyl-3-(1-piperidinyl)1-propanone hydrochloride [1]. The fixed-dose combination of PAR, ACE and ES in Acemyoset P tablet is therapeutically indicated for muscle pain. PAR, ACE, and ES concurrently were revealed. Few chemometric aided UV spectrophotometric methods [2,3,4,5,6,7] were reported for PAR and ACE in combination with other drugs. The rationale of the present work aims to develop eco-friendly, simple UV spectrophotometric and chemometric methods Principal Component regression (PCR) and

Methods

Results

Conclusion