Abstract
The main aim of these studies was the characterisation and identification of lactic acid (LAB) bacteria isolated from untreated silage, and the effect of selected bacteria (inoculant was called Silko for maize) on ensiling of maize high-moisture grain. Four isolates of L(L1, L2, L3 and L4) were characterised by the use of phenotypic assays and identified by phylogenetic analysis of 16S rRNA as L. plantarum. The fresh maize high-moisture grain was ensiled with a Silko for maize inoculant, inoculant available in the market (positive control) and no additive (untreated; negative control). After 60 days of ensiling, the results showed that the chemical composition and fermentation characteristics were better in treated silages with inoculants compared to the negative control. The contents of ash, fat and lactic acid (LA) were significantly higher in the silages treated with inoculants than in negative control. In comparison, the contents of cellulose, acid detergent fibre (ADF), neutral detergent fibre (NDF), NH3-N/total nitrogen and butyric acids (BA) were considerably lower in silage treated with Silko for maize compared to the positive control. The Silko for maize improve nutritional value and fermentation of maize grain silage and is a competitive product on the market.
Highlights
Preparation of quality silage is crucial for the profitability of livestock farms because it is a source of food for the periods of the year when animal nutrition is inadequate in terms of quantity and quality
Four Lactobacillus isolates were denoted in laboratory collection bacteria as L1, L2, L3 and L4 and selected based on their high lactic acid (LA) production
Our results showed that the silages treated with L. plantarum inoculants have significantly higher crude protein content compared to control
Summary
Preparation of quality silage is crucial for the profitability of livestock farms because it is a source of food for the periods of the year when animal nutrition is inadequate in terms of quantity and quality. Maize is a vital crop for world farmers. Maize grain is used to satisfy the energy requirement of livestock. It can be ensiled and used as an animal feed ingredient. Ensiling of moist maize grains has several advantages, such as savings on drying costs, the high nutritional value of silage, easy use. In the case of low maize grain prices on the market, the valorisation of maize is possible through animal products, and planning of the expected profit improves
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